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Clones were digested by BamHI/EcoRI and directionally ligated to pGEX-6p-1. Error-free recombinant vectors were transformed into BL21 (DE3) pLysS cells (Novagene), which was cultured subsequently on a Luria-Bertani plate. Understanding the diet of deep-diving predators can provide essential insight to the trophic structure of the mesopelagic ecosystem. Comprehensive population-level diet estimates are exceptionally difficult to obtain for elusive marine… Blackwell Science, LtdOxford, UKMMIMolecular Microbiology0950-382XBlackwell Publishing Ltd, 2005? 2005581116130Original ArticleDownregulation of subunits of complexes III and IVA. For cholesterol depletion, cells were pre- H-90; Santa Cruz Biotechnology, Santa Cruz, CA) diluted at incubated for 1 h in serum-free Dulbecco’s modified Eagle’s 1:500. Importantly, the assembly and integrity of the Oxphos complexes and the steady-state abundance of their constituent subunits remained unchanged.
DNA was analyzed by Illumina deep sequencing technology.
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To assess whether demographic declines of Arctic species at the southern limit of their range will be gradual or punctuated, we compared large-scale environmental patterns including sea ice dynamics to ringed seal (Pusa hispida…
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For cholesterol depletion, cells were pre- H-90; Santa Cruz Biotechnology, Santa Cruz, CA) diluted at incubated for 1 h in serum-free Dulbecco’s modified Eagle’s 1:500.